Bruker 800-MHz NMR (CSB)
The Bruker 800-MHz Avance II spectrometer has a room temperature TXI probe, triple axis gradients and temperature controller. The instrument is located at the Center for Structural Biology at the University of Illinois at Chicago.
Specifications
| Part | Description |
|---|---|
| Magnet | Bruker 800-MHz US² |
| Probes | 1) TXI probe with ¹H/²H/¹³C/¹⁵N pulsing and 3-axis gradients |
| 2) TCI Cryoprobe with z-axis gradients | |
| Console | Avance II |
| Software | Topspin v1.3 |
Nutation Field Strengths
| Nucleus | Power Level | pw90 | Notes |
|---|---|---|---|
| 1H (800.2MHz) | -1.0 dB | 10μs1 | Hard pulse (<100μs) |
| 13C (201.2MHz) | -5.0 dB | 11.8μs | Hard pulse (<100μs) |
| 9.1 dB | 60.0μs | Decoupling pulse (<80ms) | |
| 15N (81.08MHz) | -5.0 dB | 50μs | Hard pulse (<100μs) |
| 7.9 dB | 220μs | Decoupling pulse (<80ms) |
-
Calibrate on every sample ↩